Technical Data
C1035-27S2
CAPN1 (Calpain-1 Catalytic Subunit, Calcium-activated Neutral Proteinase 1, CANP 1, Calpain mu-type, Calpain-1 Large Subunit, Cell Proliferation-inducing Gene 30 Protein, Micromolar-calpain, muCANP, CANPL1, PIG30)
Description:
Calpain 1 (mu-calpain), a calcium-dependent cysteine C2 endopeptidase, is involved in myofibrillar protein degradation. In skeletal muscles, activation of Calpain I results in degradation of skeletal structural proteins, improving meat tenderness. Calpains I is inhibited by the endogenous inhibitor calpastatin and by leupeptin. Calpastatin is inactivated in postmortem skeletal muscle. Activity and expression of calpains in peripheral lymphoid organs may play an important role in T cell migration.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG115C10Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Calpain purified from human placenta.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 3mM sodium azide, 50% glycerol.
Specificity:
Recognizes human CAPN1. Species sequence homology: mouse, bovine, and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.