		Technical Data
C1035-27V	Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3, Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral Protease 3, New Calpain 1, NCL1, nCL-1)

Description:
Calpain 3 is a muscle-specific calcium-activated neutral protease. Defects in the gene encoding for calpain 3 appear to be the origin of a form of limb-girdle muscular dytrophy. This antibody will help detect the presence or absence of calpain 3 in western blotting and maybe useful in further characterizing calpain 3 expression.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Freshly isolated normal human skeletal muscle homogenate prepared in treatment buffer containing 0.125M tris-HCl buffer pH6.4, 10% glycerol, 4% SDS, 4M urea, 10% mercaptoethanol and 0.001% bromophenol blue (final pH of treat- ment buffer, 6.8). Bands at approx- imately 94kD and 30kD.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	Calp3d/2C4	Supernatant

Size		Storage	Shipping	Source	Host
2.5ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to aa1-19 of human calpain 3.

Purity:

Supernatant

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 2.5ml dH2O.

Specificity:

Recognizes human Calpain 3. Species Crossreactivity: Canine and rabbit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Anderson L V B, Davison K, Moss J A, et al.. “Characterization of monoclonal antibodies to calpain 3 and protein expression in muscle from patients with limb-girdle muscular dystrophy type 2A”. Am. J. Pathol. 153(4):1169-1179 (1998 ). 2.Anderson L V B. Multiplex Western blot analysis of the muscular dystrophy proteins. In Bushby K M D and Anderson L V B (eds) “Muscular Dystrophy: Methods and Protocols”, Methods in Molecular Medicine series. Topaloglu H, Dinçer P, Richard I, et al.. Calpain-3 deficiency causes a mild muscular dystrophy in childhood. Neuropediatrics. 28:212-216 (1997).