Technical Data
C1035-27V
Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3, Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral Protease 3, New Calpain 1, NCL1, nCL-1)
Description:
Calpain 3 is a muscle-specific calcium-activated neutral protease. Defects in the gene encoding for calpain 3 appear to be the origin of a form of limb-girdle muscular dytrophy. This antibody will help detect the presence or absence of calpain 3 in western blotting and maybe useful in further characterizing calpain 3 expression.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Freshly isolated normal human skeletal muscle homogenate prepared in treatment buffer containing 0.125M tris-HCl buffer pH6.4, 10% glycerol, 4% SDS, 4M urea, 10% mercaptoethanol and 0.001% bromophenol blue (final pH of treat- ment buffer, 6.8). Bands at approx- imately 94kD and 30kD.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2bCalp3d/2C4Supernatant
SizeStorageShippingSourceHost
2.5ml-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa1-19 of human calpain 3.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 2.5ml dH2O.
Specificity:
Recognizes human Calpain 3. Species Crossreactivity: Canine and rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Anderson L V B, Davison K, Moss J A, et al.. “Characterization of monoclonal antibodies to calpain 3 and protein expression in muscle from patients with limb-girdle muscular dystrophy type 2A”. Am. J. Pathol. 153(4):1169-1179 (1998 ). 2.Anderson L V B. Multiplex Western blot analysis of the muscular dystrophy proteins. In Bushby K M D and Anderson L V B (eds) “Muscular Dystrophy: Methods and Protocols”, Methods in Molecular Medicine series. Topaloglu H, Dinçer P, Richard I, et al.. Calpain-3 deficiency causes a mild muscular dystrophy in childhood. Neuropediatrics. 28:212-216 (1997).