Technical Data
C1035-27W2
CAPN2, CT (Calpain-2 Catalytic Subunit, Calpain-2 Large Subunit, Calcium-activated Neutral Proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, Calpain Large Polypeptide L2, CANPL2)
Description:
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to domain III of human Calpain-2 large subunit at C-terminus.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes human Calpain 2. It does not cross-react with other calpains. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.