		Technical Data
C1035-30E	CAPN10, NT (Calpain-10, Calcium-activated Neutral Proteinase 10, CANP 10, KIAA1845)

Description:
CAPN10 is ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. This protein is heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. The protein is similar in organization to calpains 5 and 6.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human CAPN10 (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human CAPN10.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Unsal,T., Konac,E. J. Assist. Reprod. Genet. 26(4), 205-216 (2009). 2.Garcia-Escalante,M.G. Invest Clin 50(1), 65-76 (2009). 3.Yilmaz,M., Yurtcu,E. J. Endocrinol. Invest. 32(1), 13-17 (2009). 4.Evans,J.C., Frayling,T.M. Am. J. Hum. Genet. 69(3), 544-552 (2001).