Technical Data
C1035-61B
Calprotectin (S100A8/A9, MRP-8/MRP-15) (FITC)
Description:
The calcium binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces.

Applications:
Suitable for use in Flow Cytometry, Immunoassays, Western Blot, and Immunohistochemistry (frozen and paraffin). Can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:50
Immunohistochemistry (frozen and paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG19G139Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human blood monocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes an epitope on the human S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8) or S100A9 (MRP14). Does not react with lymphocytes or platelets. Species crossreactivity: rhesus monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zwadlo, G et al; A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol 1986, 137: 512 2. Kuhn, A et al; Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus. Br J Dermatol 2002, 146: 801 3. Hessian, P et al; The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9) Antibody recognition, epitope definition and the implications for structure. Eur J Biochem 2001, 268: 353