Technical Data
C1035-61D
Calprotectin (MRP8/MRP14 Complex, S100A8/S100A9 Complex, Calgranulin A/Calgranulin B Complex)
Description:
The calcium binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. It is also a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin, cyrosections): 1ug/ml. Fix with acetone. Formalin fixed paraffin sections require pre-digestion with trypsin for 20-60 minutes.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1IDCP2Affinity Purified
SizeStorageShippingSourceHost
10ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human MRP8 (S100A8 or calgranulin A) and MRP14 (S100A9 or calgranulin B) heterocomplex.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide. Reconstitute with 10ul/100ul sterile ddH2O.
Specificity:
Recognizes human Calprotectin. Reacts with a subpopulation of macrophages, monocytes and granulocytes. Peripheral blood monocytes carry Calprotectin extra- and intracellularly, neutrophils only do so interacellularly. Species Crossreactivity: Rhesus Monkey (subpopulation of macrophages).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.