Technical Data
C1036-01D
Calretinin (Calbindin, CAB29, CAL2)
Description:
Calretinin is a neuron specific calcium binding protein (31kD) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective.

Western blot: 1:1,0001:3,000 Immunohistochemistry on rat brain tissue: IFA: 1:1,0001:5,000 PAP: 1:1,0001:5,000 The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:1001:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.
TypeIsotypeCloneGrade
MabIgG12Q43Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat calretinin
Purity:
Purified immunoglobulin.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Calretinin. Species Reactivity: Rat and Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Protein Expression and Purification (1994) 5:187191. 2) Di Cunto, F., et al., Neuron (2000) 28:115127. 3) Grazia, M., et al., J. Comparative Neurology (2001) 434:329341.