Technical Data
C1036-01G
Calretinin (Calbindin, CAB29, CAL2)
Description:
Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.

Applications:
Suitable for use in Immunohistochemistry.

Recommended Applications:
Immunohistochemistry (formalin/paraffin): 1:200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Positive Control: Mesothelioma

Cellular Localization: Nuclear and Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceMouseRabbit
Concentration:
~0.2mg/ml
Immunogen:
Recombinant full-length mouse calretinin protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.
Specificity:
Recognizes mouse Calretinin. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.