Technical Data
CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II)
Applications: Suitable for use in Immunoblot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution: Immunoblot Analysis: 0.52ug/ml detects CaM Kinase II, alpha subunit, in bovine brain cytosol and rat brain microsome preparations. Bovine brain cytosol was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CaM Kinase II (0.5ug/ml). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry: This antibody has been reported to detect CaM Kinase II, alpha subunit, in rat brain coronal sections.1

Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG14i28Affinity Purified
100ug-20CBlue IceRatMouse
Partially puri ed rat CaM Kinase II, a-subunit.
Purified by Protein G affinity chromatography.
100ug of mouse IgG1 in 100ul of 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% azide containing 30% glycerol.
Recognizes the a subunit of CaM Kinase II, Mr 50kDa, with high af nity and speci city.1 Species Crossreactivity: Rat and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Erondu, N.E. and M.B. Kennedy, J. Neurosci. 5: 32703277, 1985.