Technical Data
C1036-76A
CaM Kinase II, alpha (Calcium/calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM Kinase II Subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968)
Description:
CaMKII, one of the most abundant neuronal protein kinases was first identified in neuronal membrane fractions. CaMKII phosphorylates a broad range of substrates and is expected to have pleiotropic regulatory actions in cellular differentiation and mature neuronal functions. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha subunit of CaMKII is identified as the major postsynaptic density protein (mPSDp). It undergoes calcium/calmodulin-dependent autophosphorylation on Thr286 in its regulatory domain, rendering the kinase partially calcium-independent. Thus this Thr286 autophosphorylation controls subcellular targeting of CaMKII in neurons with important functional consequences. The protein is enriched in brain and its overexpression alters the general cell growth, differentiation, and morphology of neurons. human CaMKII gene maps on chromosome 5 at 5q33.1.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochestry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human CaMKII around the phosphorylation site of threonine 286 (Q-E-TP-V-D).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human CaMKII. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thierry, M. et al. The Journal of Neuroscience. 17:924-931 (1997). 2. Stefan, S. et al. Journal of Biological Chemistry. 272:13467-13470 (1997).