Technical Data
CaM Kinase IV (Calmodulin-Dependent Protein Kinase IV, CaMK IV, CaMKIV, CaMK-IV, Calcium/calmodulin Dependent Protein Kinase IV, Calcium/calmodulin-dependent Protein Kinase Type IV Catalytic Chain, Calcium/calmodulin Dependent Protein Kinase Type 4 Cataly
Ca(2+)/calmodulin-dependent protein kinase IV (CaMKIV) is a multifunctional protein kinase expressed abundantly in the central nervous system. Because changes in intracellular Ca(2+) concentrations affect progression through the mitotic cell cycle, enhanced expression of
CaMKIV has been reported in small cell lung carcinoma and hepatocellular carcinoma (1). Multifunctional Ca2+/calmodulin kinase IV is enriched in neurons and T lymphocytes. The kinase is absent from primary human B lymphocytes but is expressed in Epstein-Barr virus (EBV)- transformed B-lymphoblastoid cell lines, suggesting that expression of the kinase can be upregulated by an EBV gene products. In view of the key role played by LMP1 in B- lymphocyte immortalization by EBV, findings implicate Ca2+/calmodulin kinase IV as a potential mediator of B- lymphocyte growth transformation (2). Ca2+/calmodulin kinase IV can suppress the MT-regulating activity of Op18 by phosphorylation on Ser-16. These results show that Op18
phosphorylation by Ca2+/calmodulin kinase IV may couple alterations of MT dynamics in response to external signals that involve Ca2+ (3).

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:60
Western Blot: 1:100,000-1:500,000
Immunoprecipitation: 1:60
Immunohistochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues near the C-terminus of human CaMKIV.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human CaMKIV.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takai N, et al. Cancer Lett, 183(2) 185-93, 2002. 2. Mosialos G, et al. J Virol 68(3): 1697-705, 1994
3. Melander Gradin H, et al. Mol Cell Biol 17(6): 3459-67, 1997