Technical Data
Canavalia ensiformis (Concanavalin A, Jackbean) (Biotin)
Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
Isolated from Canavalia ensiformis (Jack bean) seeds Con A is one of the most widely used and well characterized lectins. Con A has broad applicability primarily because it recognizes a commonly occurring sugar structure, a-linked mannose. Since a wide variety of serum and membrane glycoproteins have a “core oligosaccharide” structure which includes a-linked mannose residues, many glycoproteins can be examined or purified with Con A and its conjugates. Con A has been utilized in hormone receptor studies, mitogenic assays, characterization of normal and malignant cells, glycoprotein purification, viral antigen isolation, dextran and mannan fractionation, cell agglutination studies, bacterial aggregation, membrane fluidity and lateral mobility investigations, turbidimetric assays for sugars, lymphokine production, as well as in many other applications.
At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits of approximately 26kD each. Below pH 5.6, Con A dissociates into active dimers of 52kD Acetylation, succinylation or other derivatizations can also produce stable forms with dimeric structures. “Native” Con A is a mixture of several forms of the lectin due to “nicks” occurring in the polypeptide chains. Although having little or no effect on the saccharide binding activity, these “nicks” in the sequence are often revealed even in the purest lectin preparations as additional bands in SDS-polyacrylamide gel electrophoresis. These hydrolytic cleavage sites appear to exist in the lectin as it occurs in the seeds and are not a function of isolation procedures.

Suitable for use in Immunodiffusion and IEP. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1mg-20°CBlue IceRabbit
Purified Canavalia ensiformis (Concanavalin A)
Delipidated antisera purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Recognizes Canavalia ensiformis (Concanavalin A)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.