Technical Data
C1103-01
Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K
Description:
The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also
known as high endothelial cells Nacetylglucosamine 6Osulfotransferase (HECGlcNAc6ST)
or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L selectin
ligands (4). It has a catalytic preference for core 2-branched mucin-type O glycans, but also has activity toward core 3 type of O glycan 5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~0.2mg/ml
Immunogen:
CHO cell-derived, recombinant human CHST1 aa24- 411.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes human CHST1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282.
2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
5. Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.
6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V,
Academic Press, Inc., New York, 964.
7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
8. Wu, Z.L., et al. (2002) Faseb J. 16:539.