Technical Data
C1103-04
Carbohydrate Sulfotransferase (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-2, GST-3, HEC-GlcNAc6ST, HEC-GLCNAC-6-ST, L-selectin ligand sulfotransfera
Description:
The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also
known as high endothelial cells Nacetylglucosamine 6Osulfotransferase (HECGlcNAc6ST)
or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L selectin
ligands (4). It has a catalytic preference for core 2-branched mucin-type O glycans, but also has activity toward core 3 type of O glycan 5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.

Length (aa): 386
Mol. Weight (Da): 45134

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.25ug/ml. Titre: ~1:300,000. Dilute antibody in 5% skim milk, PBS buffer. HRP conjugated anti-Rabbit IgG should be diluted 1: 50.000-100.000 as secondary antibody.
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
Pab
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to a region of Human CHST4
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in 2% sucrose. Reconstitution: 100ul of distilled water. Final antibody concentration is 1mg/ml.
Specificity:
Recognizes human Carbohydrate Sulfotransferase (CHST4, N-acetylglucosamine 6-O sulfotransferase 4, LSST).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282.
2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
5. Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.
6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V,
Academic Press, Inc., New York, 964.
7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
8. Wu, Z.L., et al. (2002) Faseb J. 16:539.