Technical Data
C1103-04F
Carbohydrate Sulfotransferase (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-2, GST-3, HEC-GlcNAc6ST, HEC-GLCNAC-6-ST, L-selectin ligand sulfotransfera
Description:
Human CHST4 is an N-acetylglucosamine 6-O sulfotransferase. It transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. CHST4 is a type II transmembrane protein and is localized to the Golgi. It is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Human CHST4 shares 72% aa sequence identity with the mouse ortholog.

Applications:
Suitable for use in Direct ELISA, Immunoprecipitation and Neutralization. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG111D5Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa29-386 from human CHST4 expressed in CHO cells (Q8NCG5).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute sterile PBS.
Specificity:
Recognizes human CHST4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.