		Technical Data
C1103-04F	Carbohydrate Sulfotransferase (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-2, GST-3, HEC-GlcNAc6ST, HEC-GLCNAC-6-ST, L-selectin ligand sulfotransfera

Description:
Human CHST4 is an N-acetylglucosamine 6-O sulfotransferase. It transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. CHST4 is a type II transmembrane protein and is localized to the Golgi. It is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Human CHST4 shares 72% aa sequence identity with the mouse ortholog.

Applications:
Suitable for use in Direct ELISA, Immunoprecipitation and Neutralization. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	11D5	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant corresponding to aa29-386 from human CHST4 expressed in CHO cells (Q8NCG5).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute sterile PBS.

Specificity:

Recognizes human CHST4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.