Technical Data
C1103-12
Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K
Description:
The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4 O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST1, also known as keratan sulfate Gal6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate (3). It also.has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation (4). Human CHST1 shares 94% amino acid sequence identity with mouse
CHSTI.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/ml
Neutralization: 10ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11D37Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to human CHST1 expressed in CHO cells (NP_003645).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes human CHST1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hemmerich, S. and S.D. Rosen (2000) Glycobiology 10:849. 2. Bowman, K.G. and C.R. Bertozzi (1999) Chem. Biol. 5:447. 3. Fukuta, M. et al. (1997) J. Biol. Chem. 272:32321.4. Yamada, T. et al.(2004)Biochem. J. 384: 567.