Technical Data
C1146-04
Carbonyl Reductase 1 (CBR 1, CRN, CBR, hCBR1)
Description:
Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:16,000
Western Blot: 0.2-0.5ug/ml; ~30-35kD band seen in Human Liver and Kidney lysates [Calculated MW of 30.4kD according to NP_001748.1.
Immunohistochemistry: 1-2ug/ml; In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-HGQFVSEKRVEQW, from the C Terminus of human Carbonyl Reductase 1 (NP_001748.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Carbonyl Reductase 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gonzalez-Covarrubias V, Zhang J, Kalabus JL, Relling MV, Blanco JG.
Pharmacogenetics of human carbonyl reductase 1 (CBR1) in livers from black and white donors.
Drug Metab Dispos. 2009 Feb;37(2):400-7.
PMID: 19022938
The goat polyclonal antibody used in this paper was manufactured by us:
Choi I, Kim JY, Lee EJ, Kim YY, Chung CS, Chang J, Choi NJ, Chung HJ, Lee KH.
Ontogeny of expression and localization of steroidogenic enzymes in the neonatal and prepubertal pig testes.
J Androl. 2009 Jan-Feb;30(1):57-74.
PMID: 18772489
The goat polyclonal antibody used in this paper was manufactured by us:
Gavelová M, Hladíková J, Vildová L, Novotná R, Vondrácek J, Krcmár P, Machala M, Skálová L.
Reduction of doxorubicin and oracin and induction of carbonyl reductase in human breast carcinoma MCF-7 cells.
Chem Biol Interact. 2008 Oct 22;176(1):9-18.
PMID: 18755171
The goat polyclonal antibody used in this paper was manufactured by us:
Kassner N, Huse K, Martin HJ, Gödtel-Armbrust U, Metzger A, Meineke I, Brockmöller J, Klein K, Zanger UM, Maser E, Wojnowski L.
Carbonyl reductase 1 is a predominant doxorubicin reductase in the human liver.
Drug Metab Dispos. 2008 Oct;36(10):2113-20.
PMID: 18635746
The goat polyclonal antibody used in this paper was manufactured by us:
Waclawik A, Kaczmarek MM, Kowalczyk AE, Bogacki M, Ziecik AJ.
Expression of prostaglandin synthesis pathway enzymes in the porcine corpus luteum during the oestrous cycle and early pregnancy.
Theriogenology. 2008 Jul 15;70(2):145-52.
PMID: 18439667
The goat polyclonal antibody used in this paper was manufactured by us:
Waclawik A, Ziecik AJ.
Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig.
J Endocrinol. 2007 Sep;194(3):499-510.
PMID: 17761889