Technical Data
C1209-74A
Carboxypeptidase A1 (CPA1)
Description:
CPA1 is an exopeptidase mainly produced by the pancreas. It cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. CPA1 is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The amino acid sequence of human CPA1 is 88%, 82%, and 77% identical to that of dog, rat/mouse, and chicken, respectively.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9H80Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~500ug/ml
Immunogen:
NS0-derived, recombinant human CPA1 aa17-419; NP_001859.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes human CPA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.