Technical Data
Carboxypeptidase Y
Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:10,000-1:42,000
Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Bakers yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
100ug4C (-20C Glycerol)Blue IceRabbit
Carboxypeptidase Y (yeast).
Purified by delipidation, salt fractionation and ion exchange chromatography.
Supplied as a liquid from PBS, pH 7.2, 0.01% sodium azide
Immunoelectrophoretic assay gives a single precipitin arc against anti-rabbit serum, purified carboxypeptidase Y and partially purified carboxypeptidase Y (yeast). Although not tested, the antibody should exhibit crossreactivity with other tissues and species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.