Carcinoembryonic antigen (CEA), which is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Anti-CEA is reportedly useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Immunoprecipitation (Native and denatured): 10ul/mg protein lysate. Use Protein A.
Western Blot: 1:200 for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:250-1:500. Incubate for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissue.
Optimal dilution determined by the researcher.
Recommended Positive Control:
LS174T cells or colon carcinoma
Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not Determined|
|Human CEA isolated from hepatic metastasis of colon adenocarcinoma. Cellular Localization: Cytoplasmic and lumenal membrane. MW of Antigen: 180kD.|
|Purified by ammonium sulfate precipitation, followed by ion-exchange chromatography and appropriate solid-phase absorption|
|Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.|
|Recognizes human CEA and CEA-like proteins such as non-specific crossreacting antigen (NCA), NCA2, and biliary glycoprotein (BGP1). Has been absorbed with plasma proteins and blood group antigens A and B. Species Crossreactivity: mouse. Weakly reacts with rat. Does not react with canine.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
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