Technical Data
CARD4 (Nucleotide-binding Oligomerization Domain-containing Protein 1, Caspase Recruitment Domain-containing Protein 4, NOD1)
Nod1 is implicated in the intracellular detection of pathogen associated molecule, such as, microbial peptidoglycans and signals the activation of NF-kB. Nod1 has an NH2-terminal CARD-linked to a nucleotide-binding domain and a COOH-terminal segment with multiple leucine-rich repeats, which are involved in binding to multiple caspases with long prodomains, but specifically activated caspase-9 and promoted caspase-9-induced apoptosis. Nod1 is a leucine-rich repeat-containing Apaf-1-like molecule that can regulate both apoptosis and NF-kB activation pathways.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2500
Optimal dilutions to be determined by the researcher.

Positive Control:
Recombinant fusion protein

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to aa930-949 of human Nod1.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human CARD4. Species sequence homology: mouse and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Inohara N, Koseki T, del Peso L, Hu Y, Yee C, Chen S, Carrio R, Merino J, Liu D, Ni J, Nunez G. J Biol Chem. 274 (21):14560-14567 (1999). 2. Girardin SE, Travassos LH, Herve M, Blanot D, Boneca IG, Philpott DJ, Sansonetti PJ, Mengin-Lecreulx D. J Biol Chem. 278(43):41702-8 (2003). Epub 2003 Jul 18. 3. Kim JG, Lee SJ, Kagnoff MF. Infect Immun. 72(3):1487-1495 (2004).