		Technical Data
C1340-04A	CARD 6 (CARD containing inhibitor of Nod1 and Cardiak-induced NF-kB activation, Caspase recruitment domain family, member 6, Caspase recruitment domain protein 6, CINCIN1)

Description:
This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 1ug. ~ 45-50kD and 25-30kD bands observed in A549, Human Heart and Human Muscle lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide: ATESTPSEIIERER representing the N-Terminus of the human protein (residues 2-15), according to NP_115976.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Specificity:

Species Crossreactivity: Expected to crossreact with Human due to sequence homology.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Stehlik C et al. CARD6 is a modulator of NF-kB activation by Nod1- and Cardiak-mediated pathways. J Biol Chem : (2003).