Technical Data
CARD 6 (CARD containing inhibitor of Nod1 and Cardiak-induced NF-kB activation, Caspase recruitment domain family, member 6, Caspase recruitment domain protein 6, CINCIN1)
This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 1ug. ~ 45-50kD and 25-30kD bands observed in A549, Human Heart and Human Muscle lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide: ATESTPSEIIERER representing the N-Terminus of the human protein (residues 2-15), according to NP_115976.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Species Crossreactivity: Expected to crossreact with Human due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Stehlik C et al. CARD6 is a modulator of NF-kB activation by Nod1- and Cardiak-mediated pathways. J Biol Chem : (2003).