Technical Data
CARD6 (Caspase Recruitment Domain-containing Protein 6)
CARDs (caspase recruitment domain) are homotypic protein-protein interaction motifs belonging to the death domain (DD)-fold superfamily. Homotypic interactions among CARD-containing proteins can occur with various proteins, including the inactive zymogen forms of caspases which have N-terminal pro-domains containing complementary CARDs. These interactions can result in the formation of multiprotein complexes, leading to protease cleavage and caspase activation. Most proteins containing a CARD are involved in signal transduction pathways that regulate apoptosis, adaptive immunity, or innate immunity including NF-kB activation and inflammation. There is a large number of CARD containing proteins, and the unique function of each remains to be fully elucidated. CARD6 is a CARD-containing protein with a domain structure that doesn't appear to be shared by any other protein. CARD6 has been found to associate with microtubules, and is structurally and potentially functionally related to the superfamily of interferon (INF)-inducible GTPases. INF-inducible GTPases belong to a growing family of host defense proteins that confer cell immunity. Specifically, is thought that CARD6 may act as a pattern recognition receptor that combats pathogens in a cell-autonomous manner.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Pancreas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to ATESTPSEIIERERC, from the N-terminus of the protein sequence according to NP_115976.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Recognizes human CARD6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stehlik C, Hayashi H, Pio F, Godzik A, Reed JC. CARD6 is a modulator of NF-kB activation by Nod1- and Cardiak-mediated pathways. J Biol Chem. 2003 May 29 [Epub ahead of print] PMID: 12775719.