Technical Data
CARD8 (Caspase Recruitment Domain, Cardinal/TUCAN)
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARDcontaining protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis (1,2), and caspase-1 and its related proteins ICEBERG and pesudo- ICE (3). Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein (2,4), it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-eB activation.

Can be used for detection of CARD8 by Western blot at 2ug/ml. K562 cell lysate can be used as a positive control and a band at approximately 45kD can be detected. For research use only.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to amino acids at the C-terminus of human CARD8 (Genbank accession No. AAG50014)
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Recognizes human CARD8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scholl FA, et al. J. Cell Biol. 2000; 151: 495-506. 2. Stil R, Leonardi A, et al. FEBS 2002; 521: 165-9. 3. Razmara M, et al. J. Biol. Chem. 2002; 277:13952-8. 4. Pathan N, et al. J. Biol. Chem. 2001; 276: 32220-9.