Technical Data
CARD10 (Caspase Recruitment Domain-containing Protein 10, CARD-containing MAGUK Protein 3, Carma 3, CARMA3)
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IKK complex, demonstrating its importance in the regulation of NF-kB transcription factor activation.

Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 5-10ug/ml
Western Blot: 5-10ug/ml
Immunocytochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
EL4 cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to human CARD10.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human CARD10. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang L, Guo Y, Huang W-J, et al. CARD10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-B. J. Biol. Chem. 2001; 276:21405-9. 2. Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9. 3. Fischer KD, Tedford K, Wirth T. New roles for Bcl10 in B-cell development and LPS response. Trends Immunol. 2004; 25:113-6. 4. Stilo R, Liguoro D, Di Jeso B, et al. Physical and functional interaction of CARMA1 and CARMA3 with I kinase -NF-B essential modulator. J. Biol. Chem. 2004; 279:34323-31.