Technical Data
CARD10 (Caspase Recruitment Domain-containing Protein 10, CARD-containing MAGUK Protein 3, Carma 3, CARMA3)
CARMA proteins are scaffold molecules that belong to the membrane-associated guanylate kinase-like (MAGUK) and the caspase-associated recruitment domain (CARD) protein families. They play critical role in antigen receptor signaling pathways. CARMA1, CARMA2, and CARMA3 share homologous sequence and function but are found in different tissues. CARMA1 is predominantly located in lymphoid tissue. CARMA1 protein associates with lipid rafts and interacts with Bcl10, that is required for activation of TCR induced NFkB signaling pathway. Specifically, CARMA1 N-terminal CARD domain binds to Bcl10 CARD motif. It is also essential for protein- induced IL-2 and INF-g production. CARMA genes deactivation studies in mice resulted in reduced immunoglobulin levels and absence of TCR-induced NFkB activation. This suggests that CARMA mutations may lead to adaptive and innate immunity malfunction.

Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 3-5ug/extract from 10e7 cells
Immunohistochemistry: 2.5-20ug/ml (10ug/ml-optimal)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to the internal portion of mouse CARMA3.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes mouse CARMA3. Species sequence homology: rat, canine and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gaide, O et al. (2002) Nature Immunology 3: 836-843. 2. Hara, H. et al. (2004) JEM, 200(9): 1167-1177. 3. Narayan, P. et al. (2006) Mol Cell Bio 26(6): 2327-2336. 4. Egawa T. et al. (2003) Current Biology 13: 1252-1258. 5. Grabiner, B. et al. (2007) Genes and Dev. 21: 984-996. 6. Newton, K. et al. (2003) Current Biology 13: 1247-1251. 7. Stilo R. et al. (2004) J. Biol. Chem. 279: 34323-34331.