Technical Data
C1375
Cardiolipin
Description:
Cardiolipin is an important component of the inner mitochondrial membrane, where it constitutes about 20% of the total lipid composition. The name ‘cardiolipin’ is derived from the fact that it was first found in beef heart in the early 1940s. In mammalian cells, cardiolipin (CL) is found almost exclusively in the inner mitochondrial membrane where it is essential for the optimal function of numerous enzymes that are involved in mitochondrial energy metabolism.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:100
Optimal dilutions to be determined by the researcher.
Anti-Cardiolipin Activity by ELISA: As reported (lot-specific). Current lot value: 13GPL/ml
Interpretation of Results:
>12GPL/ml: Positive
In an ELISA system designed to detect all IgG subclasses, the following purified antigens (dilution of 1:100) responded as:
Antigen Result (EU/ml)
SSA 33
SSB 9
SCL 18
Sm 7
Sm/RNP 11
Jo-1 31

Interpretation of Results:
<16 EU/ml: Negative
16-20 EU/ml: Equivocal
>20 EU/ml: Positive

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
2ml-20°CBlue IceHumanHuman
Concentration:
Not Determined
Immunogen:
Auto-antibody derived from single donor human serum.
Purity:
Purified by delipidation, defibrination and ammonium sulfate precipitation.
Form
Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity:
Recognizes human Cardiolipin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.