Technical Data
C1385-12
Cardiotrophin 1 (CT-1)
Description:
Cardiotrophin-1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT-1 was also detected in abundance in normal adult human lungs and was expressed in both fetal and adult airway smooth muscle cells.

CT-1 activates gp130-dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT-1 also has a neurotrophic function. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during the period between embryonic day 14 and the first postnatal week.

Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. CT-1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. CT-1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells. CT-1 increases ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA. CT-1 levels are significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.

Applications:
Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.

Recommended Dilution:
ELISA: To detect hCT-1 by direct ELISA (using 100l/well antibody solution) a concentration of at least 0.5g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hCT-1.
Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3.0ng/lane, under reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hCT-1 (15ng/ml), a concentration of 0.25-0.40ug/ml of this antibody is required.
Optimal dilutions to be determined by the researcher.

Reconstitution:
Reconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Highly pure (> 98%) recombinant human Cardiotrophin-1
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder.
Specificity:
Recognizes human Cardiotrophin-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.