Technical Data
C1385-26
CARMA1, NT (Bimp2, CARD11, Caspase Recruitment Domain-containing Protein 11, CARD-containing MAGUK Protein 1)
Description:
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IkK complex, thereby regulating activation of the NF-kB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: Detects a band at ~130kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
N-terminal human CARMA1 (aa 1-221 containing the CARD domain; UniProt Accession #Q9BXL7)
Purity:
Serum
Form
Supplied as a liquid.
Specificity:
Recognizes human CARMA1 at ~130kD. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. O. Gaide, et al.; Nat. Immunol. 3, 836 (2002) 2. R.B. Medeiros, et al.; Science 316, 754 (2007).