		Technical Data
C1385-26B	CARMA1 (Bimp2, CARD11)

Description:
This protein belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 1-3ug/ml. ~ 150kD band observed in lysates of cell line Jurkat (calculated MW of 133kD according to NP_115791.2).
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide: QRKTIWVDEDQL representing the C-Terminus of the human protein (residues 1131-1147), according to NP_115791.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

Specificity:

Species Crossreactivity: Human. Expected to crossreact with Mouse due to sequence homology.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Bertin J et al. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B. J Biol Chem 276:11877-82 (2001).