Technical Data
C1385-26E
CARMA1 (Bimp2, CARD11, Caspase Recruitment Domain-containing Protein 11, CARD-containing MAGUK Protein 1)
Description:
CARMA1 is a 1154aa protein playing a major role in the activation of NF-kappa-B through BCL10 and IKK. It contains a caspase-associated recruitment domain (CARD) and is thus included in the CARD protein family, a guanylate kinase-like domain and a PDZ (DHR) domain. The domain structure is similar to that of CARD14 protein and belongs to a class of proteins known as the membrane-associated guanylate kinase (MAGUK) family acting as molecular scaffolds. Binding of CARD11 and BCL10, a positive regulator of cell apoptosis and NF-kappaB activation, to each other occurs by CARD-CARD interaction and it stimulates the phosphorylation of BCL10. CARMA1 is detected in adult peripheral blood leukocytes, thymus, spleen and liver and in some cell lines except in HeLa S3, Molt-4, A549 and G431 cells.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human or mouse thymus cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to human CARMA1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human CARMA1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9. 2. Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappa B activation. FEBS Lett. 2001; 496:121-7. 3. Bertin J, Wang L, Guo Y, et al. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B. J. Biol. Chem. 2001; 276:11877-82. 4. McAllister-Lucas LM, Inohara N, Lucas PC, et al. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-kappaB induction. J. Biol. Chem. 2001; 276:30589-97. 5. Stilo R, Liguoro D, Di Jeso B, et al. Physical and functional interaction of CARMA1 and CARMA3 with I kinase -NF-B essential modulator. J. Biol. Chem. 2004; 279:34323-31. 6. Hara H, Wada T, Bakal C, et al. The MAGUK family protein CARD11 is essential for lymphocyte activation. Immunity 2003; 18:763-75.