		Technical Data
C1386-50	CART (Cocaine and Amphetamine Related Transcript, CARP)

Description:
Cocaine and Amphetamine Related Transcript (CART) was initially identified using PCR differential display as mRNA whose levels in the brain was specifically induced by psychomotor stimulants such as cocaine and amphetamine. CART encodes a secretory single chain polypeptide of 129aa or 116aa protein due to the usage of alternate splicing and processing. The first 27aa represents the hydrophobic core indicative of signal peptide. The CART protein also has several basic amino acids near the 54-66 residues with a potential to form multiple proteolytic forms. CART mRNA was specially enriched in the hypothalamus. In the striatum, CART expression was induced 4-5 fold by cocaine and amphetamine. CART is highly conserved between human and mouse (95% amino acid homology); CART gene was localized to human chr 5.
CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (3). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:50,000 using 50-100ng control peptide/well
Immunohistochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
C1386-50A: CART, aa55-102 Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Rat	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa55-102 from mouse/rat CART conjugated to KLH.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

Specificity:

Recognizes rat Cocaine and Amphetamine Related Transcript (CART). Species Crossreactivity: mouse. Species Sequence Homology: sheep; 100%, human; 97%

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gustincich S, 2004 PNAS 101 , 5069-5074, 2. Burdyga G , 2002, Neuroscience 109, 339-347 3. Burdyga G, 2004, J. Neurosci., 24: 2708 – 2715, 4. Rothman RB, 2003 Peptides 24, 413-417. General: 1. Douglass, J, et al. (1995) J. Neurosci. 15 :2471. 2. Douglass, J and Daoud S (1996) Gene 169:241. 3. Kristensen, P., et al. (1998) Nature 393:72.