Technical Data
Cartilage Intermediate-Layer Protein 1 (CILP-1)
The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725-1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively.

Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml detection limit for rhCILP-1 is ~ 1.3ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhCILP-1 is ~ 20ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
As reported
NS0-derived rhCILP-1. (aa 724 - 1184)
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Recognizes human CILP-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.