		Technical Data
C2069-40J	Catenin alpha1 (alpha-1 Catenin, CTNNA1, Catenin (Cadherin-associated Protein) alpha 1 102kDa, Catenin alpha, alpha-Catenin, alpha-E-Catenin, alphaE-Catenin, CAP102, FLJ36832, FLJ52416, HGNC:2509, NY-REN-13 Antigen, Renal Carcinoma Antigen NY-REN-13)

Description:
Alpha-catenin belongs together with beta and gamma-catenin to the catenin family. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Cadherins are single pass, type I transmembrane proteins that are localized in the adherens junction. To be fully functional, the cadherin cytoplasmic domain must be linked to the actin cytoskeleton through a group of proteins termed catenins: alpha-catenin, beta-catenin and plakoglobin. Alpha catenin interacts with the cadherin indirectly via interactions with beta-catenin or plakoglobin.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	1G5	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Human alpha-catenin.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

Specificity:

Recognizes human alpha-catenin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Nieman, M et al; Mechanism of extracellular domain-deleted dominant negative cadherins. J. Cell Science 1999, 112: 1621 2) Johnson, K et al; P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252.