Technical Data
C2069-40K
CTNNA1, phosphorylated (Ser641) (Catenin alpha-1, Catenin alpha1, Alpha E-catenin, Cadherin-associated Protein, Renal Carcinoma Antigen NY-REN-13)
Description:
Three catenin proteins, a, b and y, bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion. Not only associated with E-cadherin, a-catenin was also shown to associate with other members of the cadherin family, such as Ncadherin and P-cadherin. b-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. b-catenin has also been found in complexes with the tumor suppressor protein APC. y-catenin, also known as plakoglobin, is a protein that binds with a-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTP associates with catenin/cadherin complexes and may regulate complex signaling.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to human a-catenin around the phosphorylation site of serine 641 (D-D-SP-D-F).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human Catenin alpha1 when phosphorylated at serine 641. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.