Technical Data
C2069-40R
CTNNA1 (Catenin alpha-1, Alpha E-catenin, Cadherin-associated Protein, Renal Carcinoma Antigen NY-REN-13)
Description:
Three catenin proteins, a, b and y, bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion. Not only associated with E-cadherin, a-catenin was also shown to associate with other members of the cadherin family, such as Ncadherin and P-cadherin. b-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. b-catenin has also been found in complexes with the tumor suppressor protein APC. y-catenin, also known as plakoglobin, is a protein that binds with a-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTP associates with catenin/cadherin complexes and may regulate complex signaling.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in human colon and skin lysates on ~100kD bands
Immunohistochemistry (Paraffin): 3-6mg/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-KREKQDETQTKIK, from human CTNNA1, at the internal region of the protein (NP_001894.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human CTNNA1. Species sequence homology: Canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lynch HT, Grady W, Suriano G, Huntsman D. Gastric cancer: New genetic developments. J Surg Oncol. 2005 May 13;90(3):114-133.