Technical Data
C2069-41A
Catenin, alpha T (a-T-Catenin, Catenin alpha-3)
Description:
Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alpha-T-catenin is expressed in other tissues, the highest levels being observed in the heart. Immunohistochemical analysis showed human alpha-T-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alpha-T-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells.
The encoded protein has about the same predicted size (100kD) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alpha-T-catenin cDNA, interaction with beta-catenin was demonstrated by co-immunoprecipitation.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: Acetone-fixed cells
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells, followed by permeabilization in 0.2% Triton X-100 for 2 min.
Immunohistochemistry: 1:12.5-1:100; Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Western Blot: 1:50-1:500
Optimal dilutions to be determined by the researcher.

Hybridoma:
Derived from a fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a C57BI/6 mouse.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG2a4A21Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa164-177 of the human alpha T Catenin protein including an additional N-terminal cysteine, coupled to KLH [C-VANKSDLQKTYQKL]
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes human alpha T Catenin. Epitope is located between residues 164-177. Does not crossreact with alpha E Catenin or alpha N Catenin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Janssens, B., et al., J. Cell Sci. 114: 3177-3188 (2001).