Technical Data
Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin)
The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function. a-catenin is a 102kD protein that is associated with the cadherin indirectly through its interaction with b-catenin and g-catenin. b-Catenin and its homolog plakoglobin associate directly with the cadherin. Thus, the cell-cell adherens junction is a structure composed of the transmembrane cadherin, which is associated directly with either b-catenin or plakoglobin, which in turn assoctiates directly with a-catenin. a-Catenin then mediates the interaction between the cadherin-catenin complex and the actin cytoskeleton. a-Catenin has been characterized as a tumor suppressor, suggesting that it may have a regulatory function in regulation to its structural role.

Suitable for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml for HRP/ECL detection. Recommended Blocking buffer: Casein/Tween-20 based blocking and blot incubation buffer.
Immunoprecipitation: 1-10ug per 10e6 vanadate treated A431 cells
Immunocytochemistry: 0.1-1ug/ml; alpha catenin may tolerate formaldehyde fixation
ELISA: 0.05ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
A0001-50B: A431 Cell Lysate, untreated

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ug-20CBlue IceHumanMouse
Recombinant protein corresponding to human alpha-catenin.
Purified by thiophilic adsorption and size exclusion chromatography.
Supplied as a liquid in PBS, PEG, sucrose, 0.09% sodium azide
Recognizes human alpha-catenin. Detects a band of ~102kD. Species Crossreactivity: mouse, rat, and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.