Suitable for use in ELISA, Western Blotting. Other applications not tested.
Western Blotting: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Synthetic peptide: DLANRFKEFGKKMVKL conjugated to KLH, corresponding to amino acids 171-186 of Human alpha 2 Catenin.|
|0.01M PBS. pH 7.4|
|Anti-alpha-N-Catenin recognizes alpha-N-catenin (102kD) by immunoblotting.Staining of the alpha-N-catenin band in immunoblotting is specifically inhibited with the alpha-N-catenin immunizing peptide (human, amino acids 171-186). Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (93% identity with immunogen) and Chicken (93% identity with immunogen) due to sequence homology. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Knudsen KA et al. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol 130:67-77 (1995). <7790378>Cowin P Unraveling the cytoplasmic interactions of the cadherin superfamily. Proc Natl Acad Sci U S A 91:10759-61 (1994). <7971957>Ozawa M et al. Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule. Proc Natl Acad Sci U S A 87:4246-50 (1990). <2349235>Nagafuchi A & Takeichi M Transmembrane control of cadherin-mediated cell adhesion: a 94 kDa protein functionally associated with a specific region of the cytoplasmic domain of E-cadherin. Cell Regul 1:37-44 (1989). <2519616>|