Technical Data
Catenin, beta, active (ABC)
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. alpha-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and alpha-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Suitable for use in Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemisty (Paraffin): Detects b-Catenin in mouse embryo sections.
Immunocytochemistry: Shows positive staining in LiCl-treated 293T cells fixed with methanol.
Western Blot: 0.2-2ug/ml; A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2069-47. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Flow Cytometry: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Antigen Control Included:
A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k3C196Affinity Purified
100ug-20CBlue IceHumanMouse
Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide. Also available without sodium azide. See C2069-47A.
Recognizes the active form of human beta-catenin, dephosphorylated on Ser37 and Thr41. Non-specific proteins, of MW 65kD and 148kD, were also detected. Species Crossreactivity: mouse and rat. Does not crossreact with Drosophila. Species Sequence Homology: mouse and rat: 100%; porcine: 99/100aa; frog and chicken: 97/100aa; zebrafish: 91/100aa.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. van Noort, M., et al., J. Biol. Chem. 277: 17,901-17,905 (2002). 2. Staal, F.J.T., et al., EMBO reports 3: 63-68 (2002).