Technical Data
C2069-47
Catenin, beta, active (ABC)
Description:
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. alpha-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and alpha-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution:
Immunohistochemisty (Paraffin): Detects b-Catenin in mouse embryo sections.
Immunocytochemistry: Shows positive staining in LiCl-treated 293T cells fixed with methanol.
Western Blot: 0.2-2ug/ml; A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2069-47. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Flow Cytometry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Antigen Control Included:
A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3C196Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide. Also available without sodium azide. See C2069-47A.
Specificity:
Recognizes the active form of human beta-catenin, dephosphorylated on Ser37 and Thr41. Non-specific proteins, of MW 65kD and 148kD, were also detected. Species Crossreactivity: mouse and rat. Does not crossreact with Drosophila. Species Sequence Homology: mouse and rat: 100%; porcine: 99/100aa; frog and chicken: 97/100aa; zebrafish: 91/100aa.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. van Noort, M., et al., J. Biol. Chem. 277: 17,901-17,905 (2002). 2. Staal, F.J.T., et al., EMBO reports 3: 63-68 (2002).