Technical Data
C2069-47J
Catenin, beta, phosphorylated (Ser675)
Description:
Catenin-ß plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors (1, 2, 3). Beta-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. Beta-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3 (4). The phosphorylated beta-catenin affects the protein association of beta-catenin to E-cadherin and alpha-cateinin that occurs in the cell. This process can be reversed by dephosphorylation of beta-catenin (5).

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml detects a band ~88kD in treated mouse embryonic cell lysate and HEK293 whole cell lysate.
Immunohistochemistry: 1-2ug/ml
Immunoprecipitation: 3-5ug/extract from 10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
25ug-20°CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide (KRLpSVEL) of C-terminal portion of human Beta-catenin at the phosphorylated site of Serine 675.
Purity:
Purified by epitope affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes human Catenin-Beta at phosphorylation site of Ser675 Species Crossreactivity: rat, chicken, frog. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dale TC. (1998) Biochem J. 329: 209-23. 2. Sadot. E et al (2002) J. Cell Science 115: 2771-2780. 3. Giannini, A et al (2000) J. Biol. Chem. 275(29): 21883-21888. 4. Thilo, H et al, (2002) J. Bio. Chem. 277 (26): 2330-2335). 5. Castano J et al (2002) J. Biol. Chem. 277(35): 31541-31550.