Technical Data
C2069-50B
Catenin, beta
Description:
The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. b-catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation (Native verified): 10ug/mg Use Protein A.
Western Blot: 5-10ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF-7 cells. Breast carcinoma.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide from the C-terminus of the Beta-catenin protein. Cellular Localization: Cytoplasmic and cell membrane
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2069-50BX.
Specificity:
Recognizes human Catenin, beta at ~92kD. Species Crossreactivity: mouse, rat, chicken and Xenopus
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rimm D.L., et al(1995) Lab Invest 72(5): 590. 2. Koslov, E.R., et al. (1997) J. Biol.Chem. 272(43): 27301. 3. Hazan, R.B., et al (1997) J.Biol.Chem. 272(51): 32448–32453