Technical Data
C2069-57A
Catenin, gamma (Catenin (Cadherin-associated Protein) gamma (80kD), CTNNG, ARVD12, Desmoplakin 3, Desmoplakin-3, DP3, Desmoplakin III, Desmoplakin-III, DPIII, Gamma Catenin, Junction Plakoglobin, JUP, PDGB, PKGB)
Description:
Plakoglobin, also known as gamma-catenin belongs together with alpha- and beta catenin to the catenin family. Catenins mediates cell-cell adhesion by interaction with cadherins. Plakoglobin is found in desmosomes and adherens junctions. Plakoglobin is highly homologous to beta-catenin although its function differs from that of beta-catenin. Whereas beta-catenin has been found in potentiating hyperproliferation and tumor formation, plakoglobin can suppress tumorigenicity. Overexpression of plakoglobin has been shown to suppress cell proliferation and cell tumorigenicity in animals. Furthermore reduced plakoglobin expression has been found in tumor tissues and metastatic lesions of renal cells, esophageal carcinomas and in skin carcinomas.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10
Immunoflourescence: 1:10; fix cells with paraformaldehyde
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG115F11Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Gamma catenin
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes human gamma catenin. Species Crossreactivity: rat, weakly with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sacco, P et al; Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 1995, 34: 20201. 2. Wahl, J et al; Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci 1996, 109: 1143. 3. Lewis, J et al; Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J Cell Biol 1997, 136: 919. 4. Amitay, R et al; Reduced expression of plakoglobin correlates with adverse outcome in patients with neuroblastoma. Am J Pathol 2001, 159: 43.