Technical Data
C2086-66D
Caspase 1, CT (Caspase 1 Apoptosis-related Cysteine Peptidase, Caspase 1 Apoptosis-related Cysteine Protease, Caspase-1, CASP 1, CASP1, CASP-1, Interleukin 1 beta Convertase, IL1B Convertase, IL-1BC, IL1BC, Interleukin 1 beta Converting Enzyme, Interleuki
Description:
Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml; Detects a cleaved subunit band of ~21kD in human heart cell lysates (predicted precursor MW 45.2kD).
Immunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Human heart tissue.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
16 amino acid peptide located near human Caspase-1 carboxy terminus.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes an epitope within the C-terminal region (CT) of human Caspase-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.