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Technical Data |
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C2086-66D |
Caspase 1, CT (Caspase 1 Apoptosis-related Cysteine Peptidase, Caspase 1 Apoptosis-related Cysteine Protease, Caspase-1, CASP 1, CASP1, CASP-1, Interleukin 1 beta Convertase, IL1B Convertase, IL-1BC, IL1BC, Interleukin 1 beta Converting Enzyme, Interleuki |
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Description: Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml; Detects a cleaved subunit band of ~21kD in human heart cell lysates (predicted precursor MW 45.2kD). Immunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Optimal dilutions to be determined by the researcher. IHC Positive Control: Human heart tissue. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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