Technical Data
Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Caspase 3 is one of the key executioners of apoptosis. It is responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase 3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase 3 requires aspartic acid at the P1 position.
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:800
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:300
Immunohistochemistry (Frozen): 1:600 fixed in 3% Fromaldehyde.
Immunofluorescence (IC): 1:400
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20░CBlue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3. Species Sequence Homology: porcine, bovine and canine
Purified by Protein A and immunoaffinity chromatography.
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Recognizes cleaved human Caspase 3 (Asp175). Detects endogenous levels of theálarge fragment (17/19kD) of activated caspase-3áresulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 oráother cleaved caspases. Detects non-specific caspase sunstrates by Western Blot. Species Crossreactivity: mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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