Technical Data
C2087-22C3
Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)
Description:
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kD) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kD and 12kD, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Native verified): 2ug/mg of protein lysate (Use Protein A)
Western Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cells, Tonsil and appendix

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3F49Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant full length human Caspase 3 protein. MW of Antigen: 32kD. Cellular Localization: Predominantly Cytoplasmic with some nuclear staining.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2087-22C3X.
Specificity:
Recognizes human Caspase 3 (CPP32).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krajewska M, et al. Cancer Res 1997 Apr 15; 57(8):1605–13. 2. Mallat Z, et al. Circulation 1997 Jul 15; 96(2):424–8. 3. Hum Pathol 1997 Aug; 28(8):912–21. 4. Xerri L, Devilard E, Ayello C, Brousset P, Reed JC, Emile JF, Hassoun J, Parmentier S, Birg F 4. Cell Death Differ 1998 Dec; 5(12):1004–16. 5. Srinivasan A, Roth KA, Sayers RO, Shindler KS, Wong AM, Fritz LC, Tomaselli KJ 5. Histopathology 1998 Nov; 33(5):432–9.