Technical Data
Caspase 7, CT (Caspase-7, CASP 7, CASP7, CASP-7, Apoptosis-related Cysteine Peptidase, Apoptosis Related Cysteine Protease, Apoptotic Protease MCH3, CMH 1, CMH-1, ICE-like Apoptotic Protease 3, ICE LAP3, ICE-LAP3, MCH3)
Caspase-7, a 35kD cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) is a member of the ICE/Ced-3 subfamily. Caspase-7 is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors
Fas/APO-1 and the receptor of tumor necrosis factor (TNFR-1), coupled with the fact that
granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase
(PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B
mediated apoptosis pathways.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Does not require antigen retrieval using heat treatment prior to staining.
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Skeletal Muscle Tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanRabbit
16 amino acid peptide from human Caspase-7 carboxy-terminus.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes an epitope within the C-terminal region (CT) of human Caspase-7. Species Crossreactivity: Mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.