Technical Data
Catenin, beta
b-Catenin (~92kD) shares 70% amino acid identity with both plakoglobin (g-catenin) and the product of the Drosophila segment polarity gene armadillo. b-catenin, plakoglobin (g-catenin), and p120 cas are homologous but distinct proteins which contain between 10-13 copies of a 42-44 amino acid motif rst identi ed in the armadillo protein and referred to as armadillo repeats. These armadillo repeats mediate b-catenin’s interaction with the tumor suppressor protein APC (Adenomatous Polyposis Coli).

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen and formalin-fixed, paraffin embedded tissue sections. Epitope retrieval required.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control Tissue:
Non-neural epithelial tissue

Expected Staining Pattern: Membrane and cytoplasmic
Epitope Retrieval: Required
Enzyme Digestion: Not required

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20°CBlue IceChickenMouse
Recombinant protein corresponding to the C-terminus of chicken b-catenin.
Puri ed from mouse ascites
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Recognizes chicken b-catenin (~92kD) and does not crossreact with the related plakoglobin (~83kD) protein. Species Crossreactivity: human, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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