Technical Data
C2096-60H
Catenin beta, NT (CTNNB1, Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286)
Description:
Beta catenin plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell- cell adherens junctions (AJ) molecules, components of its degradation complex, and transcription factors. Beta-catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. Beta-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3. The phosphorylated beta-catenin will affect the protein association of beta-catenin to E-cadherin and alpha-cateinin that occurs in the cell. This process can be reversed by dephosphorylation of beta-catenin.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to human beta catenin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes human beta catenin. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dale TC. (1998) Biochem J. 329: 209-23. 2. Sadot. E et al (2002) J. Cell Science 115: 2771-2780. 3. Giannini, A et al (2000) J. Biol. Chem. 275(29): 21883-21888. 4. Thilo, H et al, (2002) J. Bio. Chem. 277(26): 2330-2335). 5. Castano J et al (2002) J. Biol. Chem. 277(35): 31541-3155.