		Technical Data
C2096-64F	CTNNB1, Phosphorylated (Ser45) (Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286)

Description:
Catenin is one of the key downstream effectors in the Wnt signaling pathway. It has been implicated to play an important role in early embryonic development and tumorigenesis. b-catenin can be destabilized by GSK-3b or other kinases by phosphorylating it at serines 33, 37, 45 and threonine 41. Mutations of these phosphorylation sites in b-catenin have been found in many tumor cell lines.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to Ser45 from human b-Catenin.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.

Specificity:

Recognizes human Phospho-b-Catenin at ~90kD. Species Crossreactivity: Mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.