Technical Data
C2096-64F
CTNNB1, phosphorylated (Ser45) (Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286)
Description:
Catenin is one of the key downstream effectors in the Wnt signaling pathway. It has been implicated to play an important role in early embryonic development and tumorigenesis. b-catenin can be destabilized by GSK-3b or other kinases by phosphorylating it at serines 33, 37, 45 and threonine 41. Mutations of these phosphorylation sites in b-catenin have been found in many tumor cell lines.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic phosphopeptide corresponding to Ser45 from human b-Catenin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes human Phospho-b-Catenin at ~90kD. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.